Instituto de Ciências Biomédicas

Universidade Federal do Rio de Janeiro 


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Capa da revista Cell Motility and the Cytoskeleton, mostrando regiões de adesão no peixe-zebra, por Manoel CostaLaboratory / Laboratório de

Muscle Differentiation and the Cytoskeleton / Diferenciação Muscular e Citoesqueleto

Room F2-019; block F - 2nd floor
Centro de Ciências da Saúde
Cidade Universitária - Ilha do Fundão
Rio de Janeiro, RJ - CEP 21949-900
Phone: (21) 3938-6429

Last update: 02/05/2018


Bioterio peixe-zebra

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Links úteis:
Programas de imagem:
Image, do National Institute of Health (NIH) dos Estados Unidos: rsb.info.nih.gov/nih-image/
    - veja sua versão em Java, que independe de plataforma, e sua versão para PC, feito pela Scion
    Corporation, fabricante de placas de aquisição.

O NIH Image suporta, com os Plug-Ins apropriados, captura TWAIN e QuickTime, sendo portanto compatível com qualquer placa de vídeo.

MRIcro: www.psychology.nottingham.ac.uk/staff/cr1/mricro.html

SpectraViewer, guia de espectros de fluorescencia da Molecular Probes/ Invitrogen: http://probes.invitrogen.com/resources/spectraviewer/

ImageTools, da University of Texas, Estados Unidos: www.ddsdx.uthscsa.edu/dig/itdesc.html

ImageBrowser, da Carl Zeiss:  http://www.zeiss.de/C12567BE0045ACF1/InhaltWWWIntern/CDBB040E9EDCD2D3C12567D3004DC896


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Publications (lista parcial):

  1. Portilho DM, Soares CP, Morrot A, Thiago LS, Butler-Browne G, Savino W, Costa ML, Mermelstein C. Cholesterol depletion by methyl-ß-cyclodextrin enhances cell proliferation and increases the number of desmin-positive cells in myoblast cultures. Eur J Pharmacol. 2012 Nov 5;694(1-3):1-12. doi: 10.1016/j.ejphar.2012.07.035. Epub 2012 Aug 19. PubMed PMID: 22921450.

  2. Pontes Soares C, Midlej V, de Oliveira ME, Benchimol M, Costa ML, Mermelstein C. 2D and 3D-organized cardiac cells shows differences in cellular morphology, adhesion junctions, presence of myofibrils and protein expression. PLoS One.2012;7(5):e38147. doi: 10.1371/journal.pone.0038147. Epub 2012 May 25. PubMed PMID: 22662278; PubMed Central PMCID: PMC3360656.

  3. Yu Y, Santos LM, Mattiace LA, Costa ML, Ferreira LC, Benabou K, Kim AH, Abrahams J, Bennett MV, Rozental R. Reentrant spiral waves of spreading depression cause macular degeneration in hypoglycemic chicken retina. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2585-9. doi: 10.1073/pnas.1121111109. Epub 2012 Jan 30. PubMed PMID: 22308470; PubMed Central PMCID: PMC3289307.

  4. Conferencia de Manoel Luis Costa - Minha memo´ria de Carlos Chagas Filho. Palestra da 9ª Semana de Museus ocorrida no IBCCF da UFRJ em maio de 2011.

  5. Possidonio A.C.B. ; Senna, M.L. ; PORTILHO, Débora Morueco ; SOARES, C. P. ; Sampaio, L.S. ; LAMAS, M. E. ; BRANCO, M. T. L. C. ; COSTA, M. L. ; Mermelstein, Claudia S. alpha-cyclodextrin enhances myoblast fusion and muscle differentiation by the release of IL-4. Cytokine (Philadelphia) (Cessou em 2000. Cont. Cytokine+), v. 55, p. 280-287, 2011.

  6. Thiago L ; Mendes FA ; PORTILHO, Débora Morueco ; Abreu, J.G. ; MERMELSTEIN, C ; ROSSI, M. I. D. ; BOROJEVIC, R. The Wnt signaling pathway regulates Nalm-16 B-cell precursor acute lymphoblastic leukemic cell line survival and etoposide resistance. Biomedicine & Pharmacotherapy, v. 64, p. 63-72, 2010.

  7. Hohn Neto, A.R. ; Zancan, P. ; Martins ER ; Salgado, L.T. ; MERMELSTEIN, C. S. ; Gomes, A.M.O. ; SOLA-PENNA, M. Filamentous actin and its associated binding proteins are the stimulatory site for 6-phosphofructo-1-kinase association within the membrane of human erythrocytes. Biochimie (Paris. Print), v. 92, p. 538-544, 2010.

  8. SOARES, C. P. ; PORTILHO, Débora Morueco ; Sampaio L. ; LAMAS, M. E. ; Morales M ; COSTA, M. L. ; Mermelstein, Claudia S. Membrane cholesterol depletion by methyl-beta-cyclodextrin enhances the expression of cardiac differentiation markers. Cells Tissues Organs (Print), v. 192, p. 187-199, 2010.

  9. Pereira ECS ; CAMPOS, L. M. ; Vannier dos Santos MA ; MERMELSTEIN, C. S. ; COSTA, M. L. Distribution of cytoskeletal and adhesion proteins in adult zebrafish skeletal muscle. Histology and Histopathology, v. 24, p. 187-196, 2009.

  10. NARCISO, M. S. ; MIETTO, B. S. ; MARQUES, S. A. ; SOARES, C. P. ; MERMELSTEIN, C. S. ; El-Cheik M. ; Martinez AM Sciatic nerve regeneration is accelerated in galectin-3 knockout mice. Experimental Neurology, v. 217, p. 7-15, 2009.

  11. Costa ML, Escaleira R, Jazenko F, Mermelstein CS. “Cell adhesion in zebrafish myogenesis: distribution of intermediate filaments, microfilaments, intracellular adhesion structures and extracellular matrix, cell motility and the cytoskeleton”, publicado previamente online na Cell Motility and the Cytoskeleton, agosto de 2008, PMID: 18680203, DOI: 10.1002/cm.20301

  12. Portilho DM, Martins ER, Costa ML, Mermelstein CS. "A soluble and active form of Wnt-3a protein is involved in myogenic differentiation after cholesterol depletion." FEBS Lett. 2007 22;581(30):5787-95 PMID: 18037380

  13. Souza AR, Kozlowski EO, Cerqueira VR, Castelo-Branco MT, Costa ML, Pavão MS. "Chondroitin sulfate and keratan sulfate are the major glycosaminoglycans present in the adult zebrafish Danio rerio (Chordata-Cyprinidae)." Glycoconj J. 2007 Dec;24(9):521-30. Epub 2007 Jun 1. PMID: 17541818

  14. Mermelstein CS, Portilho DM, Mendes FA, Costa ML, Abreu JG. "Wnt/beta-catenin pathway activation and myogenic differentiation are induced by cholesterol depletion. " Differentiation. 2007 Mar;75(3):184-92. PMID: 17359297

  15. Mermelstein CS, Martins ER, Portilho DM, Costa ML. "Association between the muscle-specific proteins desmin and caveolin-3 in muscle cells." Cell Tissue Res. 2007 Feb;327(2):343-51. Epub 2006 Oct 12. PMID: 17036228

  16. Mermelstein CS, Andrade LR, Portilho DM, Costa ML. " Desmin filaments are stably associated with the outer nuclear surface in chick myoblasts." Cell Tissue Res. 2006 Feb;323(2):351-7. Epub 2005 Sep 14. PMID: 16160856

  17. Mermelstein CS, Amaral LM, Rebello MI, Reis JS, Borojevic R, Costa ML. " Changes in cell shape and desmin intermediate filament distribution are associated with down-regulation of desmin expression in C2C12 myoblasts grown in the absence of extracellular Ca2+." Braz J Med Biol Res. 2005 Jul;38(7):1025-32. Epub 2005 Jul 4. PMID: 16007273

  18. Costa ML, Escaleira R, Cataldo A, Oliveira F, Mermelstein CS. Desmin: molecular interactions and putative functions of the muscle intermediate filament protein. Braz J Med Biol Res. 2004 Dec;37(12):1819-30. Epub 2004 Nov 17. Review. PMID: 15558188

  19. Mermelstein, CS, Portilho, DM, Medeiros, R., Matos, A.R., Lamas, M.E., Tortelote, G.G., Vieyra, A., Costa, M.L. "Cholesterol depletion by methyl-b-cyclodextrin enhances myoblast fusion and induces the formation of myotubes with disorganized nuclei." Cell and Tissue Research.319(2):289-97, 2004.

  20. Costa ML, Escaleira RC, Manasfi M, Souza LF, and Mermelstein CS "Cytoskeletal and cellular adhesion proteins in the zebrafish (Danio rerio)myogenesis " Braz J Med Biol Res., 36:1117-1120, 2003.2.PMID: 12886467

  21. Mermelstein CS, Rebello MI, Amaral LM, and Costa ML "Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation." Braz J Med Biol Res. 36(8):1111-1116, 2003.

  22. Costa ML, Escaleira RC, Rodrigues VB, Manasfi M, and Mermelstein CS “Some distinctive features of zebrafish myogenesis based on unexpected distributions of the muscle cytoskeletal proteins actin, myosin, desmin, alpha-actinin, troponin and titin." Mechanisms Of Development, 116:95-104,2002.

  23. Mermelstein CS, Guma FCR, Mello TG, Fortuna VA, Guaragna RM, Costa ML and Borojevic R. "Induction of the lipocyte phenotype in murine hepatic stellate cells: reorganisation of the actin cytoskeleton Cell Tissue Res., 306:75-86, 2001.

  24. Guma FCR, Mello TG, Mermelstein CS, Fortuna VA, Wofchuck ST, Gottfried C, Guaragna RM, Costa ML and Borojevic R. “ Intermediate filaments modulation in an in vitro model of the hepatic stellate cell activation or conversion into the lipocyte phenotypeBiochem. Cell Biol., 79: 409-417, 2001.

  25. Mermelstein CS, Costa ML, Moura Neto V and Chagas C “The cytoskeleton of the electric tissue of Electrophorus electricus, L.” Anais da Academia Brasileira de Ciências, 72(3):341-351, 2000.

  26. Firma CMG, Costa MMB, Costa ML e Lemme E. “Avaliação qualitativa e quantitativa do esvaziamento gástrico através do método videofluoroscópico” Arquivos de Gastroenterologia, 37:81-88, 2000.
  27. Costa MMB, Costa ML, Firman CMG and Mermelstein CS “Volumetric quantification of the gastric emptying: computer-based method for generation of volumetric index from fluoroscopic images” Computer Methods and Programs in Biomedicine, 65:153-161, 2001.

  28. Costa MMB, Costa ML, Firman CMG e Koch HA “Videofluoroscopia como método de avaliação do esvaziamento gástrico”, Radiologia Brasileira, 33: 95-100, 2000.

  29. Bellio, M., Oliveira, A. C. S. C., Mermelstein, C. S., Capella, M. A. M., Viola, J. P. B., Levraud, J.-P., DosReis, G. A., Previato, J. O. and Mendonça-Previato, L. "Costimulatory action of Glycoinositolphospholipids from Trypanosoma cruzi: increased interleukin 2 (IL-2) secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1" FASEB J., 13: 1627-1636, 1999.
  30. Mermelstein CS, Rodrigues APM, Einicker-Lamas M, Navarrete RE de B, Farina M and Costa ML "Distribution of F-actin, alpha-actinin, tropomyosin, tubulin and organelles in Euglena gracilis by immunofluorescence microscopy" Tissue & Cell, 30: 50-57, 1998.
  31. M.L.Costa, C.S.Mermelstein, M.M.Fróes-Ferão, C.Chagas, V.Moura Neto. "Differences in the isodesmin pattern between the electric organs of the Electrophorus electricus, L." Comparative Biochemistry and Physiology, B,119: 715-719, 1998. Superposição de imunofluorescência para núcleo (azul) e desmina (verde)
  32. C.S.Mermelstein, M.L.Costa, C.Chagas e V.Moura Neto. "Intermediate filament protein in TPA-treated skeletal muscle cells in culture" J. Muscle Res. Cell Mot., 17:199-206 (1996).
  33. M.C.R.Cordeiro, M.Benchimol, C.S.Mermelstein, L.A.Sá, M.V.C.Faria, C.Chagas e V.Moura Neto. "Desmin filaments in the electrocytes of the electric organ of the electric eel Electrophorus electricus" Cell Tissue Res., 25:559-569 (1996).
  34. M.C.S.Pereira, M.Costa, C.Chagas Filho e M.N.L.Meirelles. "Myofibrillar breakdown and cytoskeletal alterations in heart muscle cells during invasion by Trypanosoma cruzi: immunological and ultrastrutural study" J. Submicrosc. Cytol. Pathol., 25: 559-569 (1993).
  35. M.L.Costa, M.N.L.Meirelles e M.C.S.Pereira. "Trypanosoma cruzi cytoskeleton: an immunofluorescence study" Memórias do Instituto Oswaldo Cruz, (1992).
  36. C.S.Mermelstein, H.Holtzer, C.Chagas e V.Moura Neto. "Effects of the phorbol ester TPA in skeletal muscle cells in culture" An. Acad. Bras. Ci., 64: 211-212 (1992).
  37. H.Holtzer, C.Dillulo, M.L.Costa, M.Lu, J.Choi, C.S.Mermelstein, T.Scultheiss e S.Holtzer. "Striated myoblasts and multinucleated myotubes induced in non-muscle cells by myod are similar to normal in vivo and in vitro counterparts" Frontiers in Muscle Research, eds. B. Ozawa e Y.Nabeshima, pp.187-207 (1991).
  38. J.Choi, M.L.Costa, C.S.Mermelstein, C.Chagas, S.Holtzer e H.Holtzer. "MyoD converts primary dermal fibroblasts, chondroblasts, smooth muscle and retinal pigmented epithelial cells into striated mononucleated myoblasts and multinucleated myotubes" Proc. Natl. Acad. Sci. USA, 87: 7988-7992 (1990).
  39. H.Holtzer, T.Scultheiss, C.Dillulo, J.Choi, M.L.Costa, M.Lu e S.Holtzer. "Autonomous expression of the differentiation program of cells in the cardiac and skeletal myogenic lineages" Ann. N. Y. Acad. Sci., 599: 158-169 (1990).
  40. M.L.Costa, C.S.Mermelstein, V.Moura Neto e C.Chagas." Desmin expression in the electric organs ofElectrophorus electricus, L." . J. Cell Biochem. Supp., 12c: 323 (1988).

  41. M.L.Costa, V.Moura Neto e C.Chagas. "Desmin heterogeneity in the main organ of Electrophorus electricus, L." Biochimie, 70: 783-789 (1988).
  42. C.S.Mermelstein, C.Chagas e V.Moura Neto. "Alpha-actinine dans l'organe èlectrique de l'Electrophorus electricus, L." C. R. Acad. Sci. Paris, 307: 717-721 (1988).
  43. M.L.Costa, M.M.Oliveira, O.Alberti, V.M.Neto e C.Chagas. "Caracterisation de la desmine dans l'organe electrique de l'Electrophorus electricus, L." C. R. Acad. Sci. Paris, 303: 547-550 (1986).
  44. M.L.Costa, A.M.Alves, V.Moura Neto e C.Chagas.

    "The purification and characterization of desmin in the Electrophorus electricus, L." An. Acad. Bras. Sci., 57: 523 (1985).

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