Advanced Optical Microscopy Course
Curso de Microscopia Óptica Avançada
July 16-20, 2012
Rio de Janeiro, Brazil
July 16-20, 2012
Advanced Optical Microscopy Course
Curso de Microscopia Óptica Avançada
Rio de Janeiro, Brazil
Programa de Pós-graduação em Ciências Morfológicas
Universidade Federal do Rio de Janeiro
SUBMISSION DEADLINE EXTENDED!
Organizers:
Manoel Luis Costa (ICB-UFRJ),
João Menezes (ICB-UFRJ)
John Murray (University of Pennsylvania - EUA),
Clarissa Henry (University of Maine - EUA)
Ke Hu (University of Indiana - EUA)
Program:
Limited to 20 students
1) Introduction: image fundamentals: resolution, brightness, contrast, etc.
Microscopy theory: physical and geometrical optics, microscope components, contrast techniques: dark field, phase, polarization,
interference. Fluorescence. Image acquisition and storage.
2) Three-dimensional and dynamic techniques: laser confocal, multiphoton, spinning disc and slit-scan confocal, deconvolution.
3) Super-resolution and specialized techniques: TIRF, FLIM, 4pi,STED. GSD, STORM, PALM, SIM.
The course is intended for pos-doctoral or advanced graduate students. Proficiency in English is a requirement. Independent use of microscopy
for research is also expected. Students will be selected through an abridged resumee and a letter stating the reasons for attending the
course, both in English.
Schedule:
Couse announcement: until Abril 30, 2012
New application deadline: May 25, 2012
Announcement of student selection and definitive enrolment: until May 31, 2012
Registration and contact: manoelluiscosta@ufrj.br
This course will be held right before the International Cell Biology Congress , July 25-28, 2012, in Rio de Janeiro
Site updated in: 14/03/2012.
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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
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8:00 - 9:00 |
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Contrast techniques: dark field and phase contrast |
Three-dimensional imaging: laser confocal, |
Live image |
Specialized techniques: TIRF, FLIM |
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Manoel |
John |
Ke |
John |
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9:00 - 10:00 |
Opening, presentations, planning |
Demonstration |
Demonstration |
Demonstration |
Demonstration |
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Manoel |
all |
all |
all |
all |
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10:00 - 11:00 |
Microscopy theory: geometrical optics |
Contrast techniques: polarization and interference |
Three-dimensional imaging: spinning disc and slit-scan confocal |
Image acquisition and storage |
Super-resolution: 4pi, STED. GSD, STORM, PALM, SIM. |
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John |
Manoel |
Manoel |
Manoel |
John |
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11:00-12:00 |
Microscopy theory: physical optics |
Demonstration |
Demonstration |
Demonstration |
Demonstration |
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all |
all |
all |
all |
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lunch |
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1:00 - 2:00 |
Microscope components |
Fluorescence: theory |
Three-dimensional imaging: deconvolution |
4D imaging |
Students' cases |
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João |
João |
Clarissa |
Clarissa |
all |
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2:00 - 3:00 |
Demonstration |
Demonstration |
Demonstration |
Demonstration |
Demonstration |
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all |
all |
all |
all |
all |
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3:00 - 4:00 |
Koehler ilumination, Abbe experiments |
Fluorescence: pratical aspects |
Three-dimensional imaging: multiphoton |
High throughput |
Course evaluation |
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Manoel |
Clarissa |
João |
João |
all |
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4:00 - 5:00 |
Demonstration |
Demonstration |
Demonstration |
Demonstration |
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all |
all |
all |
all |
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