Manoel Luis Costa

Instituto de Ciências Biomédicas
Universidade Federal do Rio de Janeiro

participação de:
Flavio Zolessi - Institut Pasteur, Uruguai
Roberta Escaleira - Instituto de Pesquisas Biomedicas do Hospital Naval Marcílio Dias - Marinha do Brasil
Cristiano Coutinho - ICB, UFRJ

INSCRIÇÕES ENCERRADAS

Fiji: http://fiji.sc/wiki/index.php/Fiji

Michael Davidson: http://micro.magnet.fsu.edu/primer/

Nikon: http://www.microscopyu.com/articles/

Olympus: http://www.olympusmicro.com/primer/

Zeiss: http://zeiss-campus.magnet.fsu.edu/

Invitrogen: http://probes.invitrogen.com/resources/education/

Invitrogen: http://probes.invitrogen.com/resources/spectraviewer/

Curso de Microscopia completo, da Sociedade Americana de Biologia Celular (ASCB):
Microscopy Series

Artigos:
Nature Milestones in Light Microscopy

Methods for imaging thick specimens: confocal microscopy, deconvolution, and structured illumination. Murray JM. Cold Spring Harb Protoc. 2011 Dec 1;2011(12):1399-437. doi: 10.1101/pdb.top066936. PubMed PMID:22135661.

Evaluating performance in three-dimensional fluorescence microscopy. Murray JM, Appleton PL, Swedlow JR, Waters JC. J Microsc. 2007 Dec;228(Pt 3):390-405. PubMed PMID:18045334

Laser Scanning Confocal Microscopy Nathan S. Claxton, Thomas J. Fellers, and Michael W. Davidson

Cellular calcium imaging: so, what´s new? Colin Brownlee, Trends in Cell Biology, 10:451-457, 2000,

Live-cell microscopy - tips and tools. Frigault MM, Lacoste J, Swift JL, Brown CM. J Cell Sci. 2009 Mar 15;122(Pt 6):753-67. doi: 10.1242/jcs.033837. PubMed PMID: 19261845.

Lighting up cells: labelling proteins with fluorophores, Atsushi Miyawaki, Asako Sawano and Takako Kogure, Nat Cell Biol. Suppl: S1-S7, 2003,

Photobleaching and photoactivation: following protein dynamics in living cells. Lippincott-Schwartz J, Altan-Bonnet N, Patterson GH. Nat Cell Biol. Suppl:S7-14. 2003,

Single-molecule visualization in cell biology. Sako Y, Yanagida T. Nat Rev Mol Cell Biol. Suppl:SS1-5. 2003,

Illuminating adhesion complexes in migrating cells: moving toward a bright future. Webb DJ, Brown CM, Horwitz AF. Curr Opin Cell Biol.15(5):614-20, 2003

Microscope: Basics and Beyond (50 pages in PDF format; 20.7 Mbytes) Mortimer Abramowitz, Olympus

Guidelines for Best Practices in Image Processing -

Microscopy from the very beginning, Zeiss

The Clean Microscope, Zeiss

How I discovered phase contrast Nobel lecture on phase contrast microscopy Frits Zernike,

Optical Microscopy Davidson & Abramowitz,

Spinning-disk confocal microscopy - a cutting-edge tool for imaging of membrane traffic.Tool Nakano A. Cell Struct Funct. 2002 Oct;27(5):349-55. Review. PubMed PMID: 12502889.

Two-Photon Microscopy of Cells and Tissue Rubart M. Circ Res. 2004 Dec 10;95(12):1154-66. Review. PubMed PMID: 15591237

Programas de imagem:
Image, do National Institute of Health (NIH) dos Estados Unidos: rsb.info.nih.gov/nih-image/
    - veja sua versão em Java, que independe de plataforma, e sua versão para PC, feito pela Scion
    Corporation, fabricante de placas de aquisição.

O NIH Image suporta, com os Plug-Ins apropriados, captura TWAIN e QuickTime, sendo portanto compatível com qualquer placa de vídeo.

MRIcro: www.psychology.nottingham.ac.uk/staff/cr1/mricro.html

SpectraViewer, guia de espectros de fluorescencia da Molecular Probes/ Invitrogen: http://probes.invitrogen.com/resources/spectraviewer/

ImageTools, da University of Texas, Estados Unidos: www.ddsdx.uthscsa.edu/dig/itdesc.html

ImageBrowser, da Carl Zeiss:  http://www.zeiss.de/C12567BE0045ACF1/InhaltWWWIntern/CDBB040E9EDCD2D3C12567D3004DC896